SphK1 immunoexpression in AMC after LPS and DMS treatments (Aa-c). The cells treated with LPS (Ba-c) appear hypertrophic (arrows) and exhibit enhanced SphK1 immunoexpression (Bb) as evident in the merged image (Bc). In cells treated with DMS alone or with DMS + LPS (Ca-c, Da-c), the intensity of SphK1 expression appears to be unaltered, compared to that of control (Aa-c). Nuclei are stained with DAPI. Scale bar = 50 μm.