Figure 5
From: Delayed inflammatory mRNA and protein expression after spinal cord injury
mRNA and protein expression profile of progranulin after spinal cord injury. Expression of progranulin was identified in the microarray and followed the expression profile of the delayed-expression cluster. Expression of progranulin peaked at 7 days post-injury and remained up-regulated through the end of the study (A). Protein expression was confirmed using immunohistochemistry at 28 days post-injury. Sham-injured tissue showed no progranulin positive cells at the lesion site (B). Injured tissue, however showed a large number of progranulin-positive cells (B). Double-labeling with Iba-1 (C) and NeuN (D) showed correlation of expression in microglia and neurons. Size bar = 200 μm (B), 100 μm (C, D).