Effect of 2,4-bis( p -hydroxyphenyl)-2-butenal on viability of astrocytes and microglial BV-2 cells. Cell viability was evaluated using a WST-8 assay as described in Methods. Astrocytes (A) and microglial BV-2 cells (B) were incubated with 2,4-bis(p-hydroxyphenyl)-2-butenal (1-10 μM) in the absence of LPS for 72 h. Astrocytes (C) and microglial BV-2 cells (D) were incubated with 2,4-bis(p-hydroxyphenyl)-2-butenal (10 μM) in the presence of LPS (1 μg/ml) for 96 h. Results are given as a percentage of viable cells related to untreated controls. The data represent the mean ± S.E. for three independent experiments performed in triplicate.