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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Inhibitory effect of a tyrosine-fructose Maillard reaction product, 2,4-bis(p-hydroxyphenyl)-2-butenal on amyloid-β generation and inflammatory reactions via inhibition of NF-κB and STAT3 activation in cultured astrocytes and microglial BV-2 cells

Figure 6

Expression of activation markers (GFAP for astrocytes and Iba1 for microglia) and Aβ 42 in astrocytes and in microglial BV-2 cells, observed by double-fluorescence. Confocal microscope observation was performed as described under in Methods. Cultured astrocytes were incubated with anti-GFAP and anti-Aβ42 primary antibodies (A), and the microglial BV-2 cells were incubated with anti-Iba1 and anti-Aβ42 primary antibodies (B). Fluorescence was developed using Alexa 568-conjugated anti-rabbit and Alexa 488-conjugated anti-mouse secondary antibodies. Images of astrocytes double-labeled with GFAP (red) and Aβ42 (green) antibodies show the fluorescent antibody staining separately and merged. Images of microglial BV-2 cells double-labeled with Iba1 (red) and Aβ42 (green) antibodies show the fluorescent antibody staining separately and merged.

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