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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Inhibitory effect of a tyrosine-fructose Maillard reaction product, 2,4-bis(p-hydroxyphenyl)-2-butenal on amyloid-β generation and inflammatory reactions via inhibition of NF-κB and STAT3 activation in cultured astrocytes and microglial BV-2 cells

Figure 7

Effects of 2,4-bis( p -hydroxyphenyl)-2-butenal on STAT3 activation in cultured astrocytes and in microglial BV-2 cells. The cells were treated with 1 μg/ml of LPS alone, or with LPS plus different concentrations (1, 5, 10 μM) of 2,4-bis(p-hydroxyphenyl)-2-butenal at 37°C for 1 h. Equal amounts of total proteins (40 μg/lane) were subjected to 10% SDS-PAGE, and activation of STAT3 (phosphorylation) was detected by western blotting using specific antibodies in astrocytes (A) and in microglial BV-2 cells (B). To block the STAT3 pathway, cells were treated with the STAT3 inhibitor AG490 (50 μM) or with 50 nM siRNA for STAT3 for 1 h prior to treatment with 2,4-bis(p-hydroxyphenyl)-2-butenal. Effect of 2,4-bis(p-hydroxyphenyl)-2-butenal on NF-κB DNA binding activity in astrocytes (C) and microglial BV-2 cells (D), and Aβ42 level in astrocytes (E) and microglial BV-2 cells (F). For determination of Aβ1-42 levels, cells were treated with 1 μg/ml of LPS alone, or with LPS plus different concentrations (1, 5, 10 μM) of 2,4-bis(p-hydroxyphenyl)-2-butenal at 37°C for 24 h. Values are mean ± S.E. for three experiments performed in triplicate. * indicates significantly different from LPS treated group (P < 0.05).

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