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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Comparing the immunosuppressive potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells

Figure 5

Detection of CD25, FoxP3, and constitutive IL-10 in T cells co-cultured with MSCs or SB623 cells in the presence of exogenous IL-2. Human T cells were co-cultured with MSCs or SB623 cells plus hIL-2 for 7 days. A) CD4 and CD25 surface expression with representative FACS data (left) and the mean expression for 5 different matched lots of MSC and SB623 cells (right); For "Donor 1 PBL", there is a significant increase in CD4+CD25+ cells when T cells were co-cultured with SB623 cells versus parental MSCs (*p < 0.05). B) The percentage of FoxP3-positive T cells as measured by intracellular staining with PE-conjugated antibody against FoxP3 followed by flow cytometry acquisition and analysis. The bar graphs represent the mean percentage of FoxP3-expressing T cells after co-culture without or with 3 different matched lots of MSC and SB623 cells. C) To assess the basal constitutive expression of IL-10, BrefeldinA (1:1000) was added during the last 6 hours of culture to inhibit the secretory pathway. By intracellular staining with a fluorochrome conjugated antibody against IL-10 and analyzed by flow cytometry. Shown here is a representative flow cytometry data data (left) and the mean expression for 3 different matched lots of MSC and SB623 cells (right) looking at the percentage of cells staining positive for intracellular IL-10 protein.

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