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Table 4 qPCR confirmation of age-related induction of MHC II pathway component genes

From: Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment

Gene

All Aged vs. Adult (ratio, p-value t-test)

Pearson Correlation to Morris water maze

Aged Intact vs. Adult (ratio, p-value SNK)

Aged Impaired vs. Adult(ratio, p-value SNK)

Aged Impaired vs.

Aged Intact(ratio, p-value SNK)

 

Syn

CA1

CA3

DG

Syn

CA1

CA3

DG

Syn

CA1

CA3

DG

Syn

CA1

CA3

DG

Syn

CA1

CA3

DG

MHC II Components

                   

Cd74

6.58###

5.61###

3.61###

2.43###

-

-

-

-

6.79***

5.66***

3.88***

2.43***

6.35***

5.58***

3.40***

2.49***

-

-

-

-

Ctse

1.69##

1.45##

1.75###

1.48###

-

-

-

-

1.81*

-

1.73***

1.48*

-

-

1.77***

1.54**

-

-

-

-

Hla-dmb

1.64###

1.37###

1.37###

1.26##

-

-

-

-

1.57**

1.33**

1.39***

-

1.72***

1.40***

1.35***

1.33**

-

-

-

1.16*

Hla-dra

6.30###

4.04###

3.02###

-

-

-

-

-

6.76***

4.18***

3.25***

-

5.75**

3.95***

2.85***

-

-

-

-

-

Hla-drb1

10.50###

7.13###

4.39###

2.91#

-

-

-

-

10.70***

7.12***

4.77***

-

10.26***

7.13***

4.12***

3.42**

-

-

-

-

Antigen Recognition

                   

C1s

1.84#

1.21#

1.56###

-

-

-

-

-

-

-

1.59**

-

-

-

1.53***

-

-

-

-

-

C3

3.47###

2.00###

1.96###

1.69###

-

-

-

-

3.40**

2.07***

2.22***

1.52***

3.55**

1.94***

1.78***

1.81***

-

-

-

1.19*

C4a

4.18###

1.77###

1.51###

-

-

-

-

-

3.83**

1.67***

1.58***

-

4.59**

1.85***

1.47***

-

-

-

-

-

Serping1

2.71###

2.03###

2.27###

1.54###

-

-

-

-

2.79***

2.18***

2.52***

1.49**

2.63***

1.92***

2.09***

1.58***

-

-

-

-

Tlr2

1.64##

-

1.54##

-

-

-

-

-

1.55*

-

1.66*

-

1.73*

-

1.46*

-

-

-

-

-

Tlr4

1.51##

1.35#

1.96###

-

-

-

-

-

1.41*

-

2.00***

-

1.60*

-

1.93***

-

-

-

-

-

Tlr7

1.69##

1.34##

1.73###

-

-

-

-

-

1.65*

1.34*

1.74***

-

1.73*

1.35*

1.72***

-

-

-

-

-

Immune Cell Activating

                  

Ccr5

1.96#

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Erbb3

1.28###

1.34###

1.20#

1.40#

-

-

-

-

1.26**

1.43***

-

-

1.30**

1.28**

-

1.47**

-

-

-

-

Fcgr2a

1.45#

1.19#

1.30###

 

-

-

-

-

-

-

1.30**

-

-

-

1.30**

-

-

-

-

-

Fcgr2b

1.96##

-

1.61###

-

-

-

-

-

2.08*

-

1.63***

-

1.82*

-

1.60***

-

-

-

-

-

Inflammation Response

                  

Cp

1.67#

-

1.56###

-

-

-

-

-

1.92*

-

1.64***

-

-

-

1.50***

-

-

-

-

-

Hsbp1

2.87###

1.68###

2.09###

-

-

-

-

-

3.13**

1.65**

2.25***

-

2.58***

1.70**

1.97***

-

-

-

-

-

Icam1

1.46#

1.55###

1.43###

-

-

-

-

-

-

1.68***

1.64***

-

-

1.46**

1.31**

-

-

-

-

-

Lgals3

3.42##

2.64###

1.98###

1.84##

-

-

-

-

3.68***

2.62***

2.18***

1.63*

3.03***

2.66***

1.84***

2.00**

-

-

-

-

S100a6

1.52#

2.03###

2.21###

-

-

-

-

-

-

2.00***

2.48***

-

-

2.06***

2.02***

-

-

-

-

-

  1. qPCR confirmation of transcriptomic findings was performed in hippocampal synaptosomes and expanded to include hippocampal subregion dissections. Age-based comparisons (aged intact and aged impaired versus adult) were statistically analyzed by two-tailed t-test with BHMTC (#p < 0.05; ##p < 0.01; ###p < 0.001). Three-group data (adult, aged intact, aged impaired) were statistically analyzed by one-way ANOVA with BHMTC, and pairwise comparisons were evaluated by SNK post hoc testing to identify potential regulation of gene expression with cognitive decline (*p < 0.05; **p < 0.01; ***p < 0.001). Correlation of gene expression with Morris water maze performance was assessed by Pearson product moment analysis with BHMTC, which demonstrated no significant association of MHC II pathway gene expression and cognitive impairment. Data are presented as ratios of group means. Individual group gene expression data is presented in Table 5.
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Journal of Neuroinflammation

ISSN: 1742-2094