Skip to main content

Table 4 qPCR confirmation of age-related induction of MHC II pathway component genes

From: Concurrent hippocampal induction of MHC II pathway components and glial activation with advanced aging is not correlated with cognitive impairment

Gene All Aged vs. Adult (ratio, p-value t-test) Pearson Correlation to Morris water maze Aged Intact vs. Adult (ratio, p-value SNK) Aged Impaired vs. Adult(ratio, p-value SNK) Aged Impaired vs.
Aged Intact(ratio, p-value SNK)
  Syn CA1 CA3 DG Syn CA1 CA3 DG Syn CA1 CA3 DG Syn CA1 CA3 DG Syn CA1 CA3 DG
MHC II Components                    
Cd74 6.58### 5.61### 3.61### 2.43### - - - - 6.79*** 5.66*** 3.88*** 2.43*** 6.35*** 5.58*** 3.40*** 2.49*** - - - -
Ctse 1.69## 1.45## 1.75### 1.48### - - - - 1.81* - 1.73*** 1.48* - - 1.77*** 1.54** - - - -
Hla-dmb 1.64### 1.37### 1.37### 1.26## - - - - 1.57** 1.33** 1.39*** - 1.72*** 1.40*** 1.35*** 1.33** - - - 1.16*
Hla-dra 6.30### 4.04### 3.02### - - - - - 6.76*** 4.18*** 3.25*** - 5.75** 3.95*** 2.85*** - - - - -
Hla-drb1 10.50### 7.13### 4.39### 2.91# - - - - 10.70*** 7.12*** 4.77*** - 10.26*** 7.13*** 4.12*** 3.42** - - - -
Antigen Recognition                    
C1s 1.84# 1.21# 1.56### - - - - - - - 1.59** - - - 1.53*** - - - - -
C3 3.47### 2.00### 1.96### 1.69### - - - - 3.40** 2.07*** 2.22*** 1.52*** 3.55** 1.94*** 1.78*** 1.81*** - - - 1.19*
C4a 4.18### 1.77### 1.51### - - - - - 3.83** 1.67*** 1.58*** - 4.59** 1.85*** 1.47*** - - - - -
Serping1 2.71### 2.03### 2.27### 1.54### - - - - 2.79*** 2.18*** 2.52*** 1.49** 2.63*** 1.92*** 2.09*** 1.58*** - - - -
Tlr2 1.64## - 1.54## - - - - - 1.55* - 1.66* - 1.73* - 1.46* - - - - -
Tlr4 1.51## 1.35# 1.96### - - - - - 1.41* - 2.00*** - 1.60* - 1.93*** - - - - -
Tlr7 1.69## 1.34## 1.73### - - - - - 1.65* 1.34* 1.74*** - 1.73* 1.35* 1.72*** - - - - -
Immune Cell Activating                   
Ccr5 1.96# - - - - - - - - - - - - - - - - - - -
Erbb3 1.28### 1.34### 1.20# 1.40# - - - - 1.26** 1.43*** - - 1.30** 1.28** - 1.47** - - - -
Fcgr2a 1.45# 1.19# 1.30###   - - - - - - 1.30** - - - 1.30** - - - - -
Fcgr2b 1.96## - 1.61### - - - - - 2.08* - 1.63*** - 1.82* - 1.60*** - - - - -
Inflammation Response                   
Cp 1.67# - 1.56### - - - - - 1.92* - 1.64*** - - - 1.50*** - - - - -
Hsbp1 2.87### 1.68### 2.09### - - - - - 3.13** 1.65** 2.25*** - 2.58*** 1.70** 1.97*** - - - - -
Icam1 1.46# 1.55### 1.43### - - - - - - 1.68*** 1.64*** - - 1.46** 1.31** - - - - -
Lgals3 3.42## 2.64### 1.98### 1.84## - - - - 3.68*** 2.62*** 2.18*** 1.63* 3.03*** 2.66*** 1.84*** 2.00** - - - -
S100a6 1.52# 2.03### 2.21### - - - - - - 2.00*** 2.48*** - - 2.06*** 2.02*** - - - - -
  1. qPCR confirmation of transcriptomic findings was performed in hippocampal synaptosomes and expanded to include hippocampal subregion dissections. Age-based comparisons (aged intact and aged impaired versus adult) were statistically analyzed by two-tailed t-test with BHMTC (#p < 0.05; ##p < 0.01; ###p < 0.001). Three-group data (adult, aged intact, aged impaired) were statistically analyzed by one-way ANOVA with BHMTC, and pairwise comparisons were evaluated by SNK post hoc testing to identify potential regulation of gene expression with cognitive decline (*p < 0.05; **p < 0.01; ***p < 0.001). Correlation of gene expression with Morris water maze performance was assessed by Pearson product moment analysis with BHMTC, which demonstrated no significant association of MHC II pathway gene expression and cognitive impairment. Data are presented as ratios of group means. Individual group gene expression data is presented in Table 5.