LPS induce up-regulation of LRP-1 expression in brain pericytes. Primary brain pericytes were stimulated for 24 h with LPS (0.1 and 1 ug/ml). After 24 h, expression of both LRP-1 subunits was analyzed by western blot as described in the Material and methods. LPS at 1 ug/ml concentration induced significant increases in expression of the large (515 kDa) and small (85 kDa) subunits of LRP-1. A representative western blot (A) and density quantification (B) based on ratios between the antibody signal (LRP-1 85 or 515 kDa) and total protein loading per lane (SYPRO) is shown. Lane designation: 1-PEA13 (LRP-1 knockout as negative control), 2-MEF1 (LRP-1 wild type as positive control), 3-CTRL, 4-LPS 0.1 ug/ml, 5-LPS 1 ug/ml. Values represent the mean ± SEM of two independent experiments * P < 0.05 vs. untreated cells, n = 5.