Figure 2

Enhanced anti-inflammatory/type I IFN gene expression but comparable pro-inflammatory gene expression in LPS-preconditioned mice post MCAO. Gene regulation 24 hr post MCAO measured by qtPCR reveals that anti-inflammatory/type I IFN-associated genes TGFβ, RANTES, and IFIT1 are significantly upregulated in LPS preconditioned mice compared to saline. Pro-inflammatory genes IL6, IL1β, COX2, and TNFα show similar regulation in LPS and saline preconditioned mice. These results confirm the gene microarray data. Samples from mice receiving a 45 (LPS: 0.2 mg/kg) or 60 min (LPS: 0.8 mg/kg) MCAO were combined due to comparable gene regulation (see methods). ND = not detected. Student's t-test, LPS vs. saline 24 hr post MCAO, **p < 0.01, n ≥ 4 per treatment.