NFκB is suppressed 24 hr post MCAO in LPS-preconditioned mice. (A) Nuclear protein obtained from ipsilateral cortices was used to measure p65 activity by EMSA analysis. EMSA gel of pooled samples (n = 4) following 45 min MCAO for saline and LPS preconditioned (0.8 mg/kg) mice (Lt.). Quantification of band intensity of individual mice following MCAO (Rt.). NFκB is significantly decreased in LPS-preconditioned mice 24 hr post MCAO compared to saline. Supershift assay confirmed specificity for p65 oligos (data not shown). (B) Ship1 and Tollip mRNA are significantly upregulated 24 hr post 60 minute MCAO in LPS-preconditioned (0.8 mg/kg) mice compared to saline, n ≥ 4 per treatment/timepoint. (C) Western blot for Ship1 and Tollip and relative band quantification showing significant upregulation of Ship1 and Tollip protein 24 hr post 45 min MCAO in LPS-preconditioned mice (0.8 mg/kg), n ≥ 3 per treatment/timepoint. (D) Western blot and relative band quantification for p105 at 24 hr post 45 minute MCAO showing significant upregulation in LPS-preconditioned (0.8 mg/kg) mice, n ≥ 3 per treatment/timepoint. (A) Two-Way ANOVA, Bonferroni Post Hoc, *p < 0.05. (B-D) Student's t-test, LPS vs. saline, **p < 0.01.