Table 1 Primer sequences used in RT-qPCR
Gene | Accession number | Primer sequences from 5' to 3' | Location of amplicon | Amplicon length | Efficiency |
|---|---|---|---|---|---|
AQP4 | Isoform 1: NM_012825.3 | Sens: TTGGACCAATCATAGGCGC | 770 to 788 Isoform 1 | 213 pb | 98.2% |
778 to 796 Isoform 2 | |||||
Isoform 2: NM_001142366.1 | Revs: GGTCAATGTCGATCACATGC | 963 to 982 Isoform 1 | |||
971 to 990 Isoform 2 | |||||
GFAP | NM_017009.2 | Sens: GCGGCTCTGAGAGAGATTCG | 692 to 711 | 90 pb | 102.0% |
Revs: TGCAAACTTGGACCGATACCA | 761 to 781 | ||||
IL1β | NM_031512.2 | Sens: AATGACCTGTTCTTTGAGGCTGAC | 111 to 134 | 115 pb | 91.2% |
Revs: CGAGATGCTGCTGTGAGATTTGAAG | 201 to 225 | ||||
GAPDH | NM_017008.3 | Sens: TGCTGGTGCTGAGTATGTCGTG | 337 to 358 | 101 pb | 89.5% |
Revs: CGGAGATGATGACCCTTTTGG | 417 to 437 |