Figure 1

Regulatory B-cells and regulatory T-cells control EAE independent of each other - B-cell depletion-associated EAE exacerbation correlates with enhanced TNF secretion of CD11b ⁺ cells. C57Bl/6 mice were injected with 200 μg anti-CD20 and/or 500 μg anti-CD25 and/or the respective isotype control in the combinations indicated prior to immunization with MOG p35-55. a) Shown are mean group scores of EAE severity ± SEM (5 mice/group). b) Depletion of B-cells and/or regulatory T-cells was evaluated by FACS staining for B220 (upper panel) or CD25/FoxP3 (lower panel, gated on CD4⁺). Shown are FACS stainings of inguinal lymph node cells obtained from representative mice prior to immunization. c) Secretion of TNF by splenic CD11b⁺ monocytes upon stimulation with LPS was evaluated by ELISA (mean of triplicates ± within subject standard deviation; 2 mice/group).