Metabolic alterations are exacerbated in rats exposed to traumatic axonal injury with additional hypoxia. Cerebral microdialysis samples were analysed between 21 h and 99 h after sham surgery, TAI and TAI with 30 min hypoxia (TAI+Hx). Data are expressed as both raw values and percentage changes from sham values for glucose (A, raw values; B, % change from sham levels), lactate (C, raw values; D, % change from sham levels) and glutamate (E, raw values; F, % change from sham). Shaded area in (C) and (D) represents the peak period of edema, which correlated with maximal lactate production. Overall a significant hypoglycaemic response was observed in both the TAI and TAI+Hx groups compared to shams (2-way repeated measures ANOVA, p < 0.05). Data shown as mean ± SEM, n = 5 per group per time point. Data was analysed by 2-way ANOVA repeated measures, with a p-value of < 0.05 considered significant.