CCL20 expression is observed in the areas of neurodegeneration of cortex and hippocampus 48 hours after TBI. A. High magnification photomicrographs of brain sections from animals subjected to TBI and sacrificed 24 or 48 h post-impact were stained with Fluoro-Jade or anti-CCL20 antibody. Fluoro-Jade staining was observed in the cortex and in the hippocampal CA1 and CA3 pyramidal cell layers 24 and 48 hours after TBI. While no CCL20 immunoreactivity was observed in the same regions of adjacent sections 24 h after TBI, CCL20 immunoreactivity was observed in the cortical neurons as well as within the hippocampal CA1 and CA3 pyramidal cell layers at 48 h. FJ, Fluoro Jade. Scale bar 50μ. B. Representative photomicrographs showing the FJ - CCL20 double staining in the cortex. CCL20 immunoreactivity was observed in most of the degenerating neurons (FJ positive) as indicated by arrows. CCL20 immunoreactivity was also observed in other cells those were not FJ positive. Scale bar 100μ.