Figure 5

Representative Luxol fast blue histochemical staining for myelin in coronal sections of spinal cords from vehicle- and diazoxide-treated mice (A and C, respectively). Quantification of the percentage of white matter area not stained by LFB shows a decrease in demyelination in diazoxide-treated mice (E). This effect was significant in the thoracic region and when the spinal cord was analyzed globally. H&E staining shows typical cell infiltrations and tissue lesions in spinal cords of vehicle- and diazoxide-treated animals (B and D, respectively). Upon quantification, results show a decrease of inflammatory lesions in all spinal cord regions (F). Results are expressed as mean ± SEM. n (animals) per group ≥ 6. Slices analyzed per animal and section ≥ 3. * p < 0.05, **p < 0.01. Scale bar = 100 μm.