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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

Figure 6

Representative images of GFAP immunostaining of spinal cord sections from vehicle- and diazoxide-treated EAE mice (A and B, respectively). Upon quantification of fluorescent signal in the gray matter, results show a decrease of GFAP intensity in cervical and thoracic region and when the spinal cord was globally analyzed in diazoxide treated animals (C). CD11b immunolabeling of spinal cord sections of vehicle- and diazoxide-treated EAE mice (D and E, respectively) allows the identification of reactive microglia/macrophages in white matter as regions with ameboid-shaped cells (D bottom, image magnification) in contrast to the resting state (E bottom, image magnification). Upon quantification, results show a smaller area of reactivity in diazoxide-treated mice when compared to vehicle treated EAE mice (F). This effect was statistically significant when the thoracic level and the spinal cord was globally analyzed. Results are expressed as mean ± SEM. n (animals) per group ≥ 4 for GFAP immunoreactivity and n (animals) per group ≥ 6 for area of microglia/macrophages reactivity. Slices analyzed per animal and section ≥ 3. *p < 0.05, **p < 0.01. Scale bar = 100 μm.

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