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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Soybean-derived Bowman-Birk inhibitor inhibits neurotoxicity of LPS-activated macrophages

Figure 4

Effect of BBI on LPS-mediated cytokine expression. Macrophages were preincubated with or without BBI (100 μg/mL) for 24 h and then treated with LPS (100 ng/mL) for an additional 24 h. (A-D) Total RNA extracted from the cells was subjected to real time RT-PCR mRNAs for cytokine (TNF-α, IL-6, IL-10 and IL-1β) mRNA expression. Data are expressed as mean ± SD for three independent experiments. (*P < 0.05, **P < 0.01, LPS + BBI treatment vs LPS treatment only). (E,F) Supernatants from macrophage cultures with indicated treatments were collected for ELISA measurement of protein levels of TNF-α and IL-6. Data are expressed as mean ± SD for three independent experiments. (*P < 0.05, **P < 0.01, LPS + BBI treatment vs LPS treatment only).

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