Figure 3
From: Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid β
PARP-1 deficiency reduces microglial activation but not Aβ accumulation in hAPP J20 mice. Microglia in cortex of 6-month old hAPPJ20 and hAPPJ20 /PARP-1 -/- mouse are immunostained with Iba-1 (green). The microglia had less activated morphology in the hAPP J20 /PARP-1 -/- brains, in both non-plaque areas (A) and in microglia contacting Aβ plaques (3D staining, red) (C). Dotted line shows the margins of plaque area. Arrows show microglia with differing morphologies; amoeboid, lacking visible processes in hAPPJ20 brain, and non-amoeboid with visible processes in the hAPPJ20/PARP-1 -/- brain. B, Quantification of microglia activation without plaque contact is presented in stacked columns presenting the scores for both microglial number and morphology. D, Morphological quantification of microglia contacting plaques. * p < 0.05; # p < 0.05, versus wt. n = 9-11. E, ELISA measurements of Aβ1-42 and Aβ1- X. n = 8-12.