Skip to main content
Figure 5 | Journal of Neuroinflammation

Figure 5

From: Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid β

Figure 5

Microglial activation by Aβ requires microglial PARP-1 activity. A. Phase contrast images of cultured microglia. 24 hour incubation with 5 μM Aβ induces morphological transformation to the activated, amoeboid morphology in wild-type (wt) microglia. Microglial activation induced by Aβ was blocked by the PARP-1 inhibitor PJ34 (PJ, 400 nM) and in PARP-1 -/- microglia. Cells treated with the reverse sequence Aβ peptide (rAβ, 5 μM) remained in resting, ramified morphology. B, Immunostaining for poly(ADP-ribose), the enzymatic product of PARP-1, showed poly(ADP-ribose) production within 60 minutes of Aβ addition, blocked by PJ34. There was no poly(ADP-ribose) signal in PARP-1 -/- cells (not shown). C. Quantification of microglial activation. D, Quantification of poly(ADP-ribose) immunoreactive cells. * p < 0.05, # p < 0.05 vs. control. n = 3.

Back to article page