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Figure 9 | Journal of Neuroinflammation

Figure 9

From: Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid β

Figure 9

PARP-1 abrogation does not impair microglial phagocytosis of Aβ. A, Phase contrast and fluorescence images show the same field of microglia cultures. FAM-labeled Aβ (5 μM, green) is internalized by both wt and PARP-1 -/- microglia, with or without a PARP-1 inhibitor (PJ34, 400 nM). B, Quantification of microglial FAM-Aβ phagocytosis activity, assessed after a 24 h exposure (n = 3). C, Orthogonal confocal views of microglia (stained with Iba-1, red) and FAM-Aβ (green) after 24 h incubation. The PARP-1 -/- microglia do not assume a reactive morphology, but nevertheless engulfed the FAM-Aβ (yellow merge).

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