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Table 3 Cytokine levels in microglia cultures

From: Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid β

 

wt microglia

PARP-1 -/-microglia

Cytokine

Basal

concentration

Fold change with treatment

Basal

concentration

Fold change with treatment

  

Aβ

Aβ+PJ34

 

Aβ

Aβ+PJ34

KC

0.78 ± 0.27

3.03 ± 1.5#

1.77 ± 0.67

0.39 ± 0.1*

1.17 ± 0.19†

1.08 ± 0.09

RANTES

1.46 ± 0.32

2.26 ± 0.86#

1.4 ± 0.27

0.76 ± 0.1*

1.69 ± 0.29#

2.36 ± 0.83

MCP-1

2.58 ± 1.07

1.81 ± 0.25#

1.15 ± 0.07†

1.06 ± 0.47

0.94 ± 0.18†

1.16 ± 0.33

MIP-1β

60.6 ± 41.9

1.91 ± 0.20#

1.44 ± 0.33

18.9 ± 4.6*

1.77 ± 0.28#

1.68 ± 0.19

IP-10

326 ± 113

0.36 ± 0.12

0.56 ± 0.10

43.4 ± 2.2*

0.48 ± 0.07

0.68 ± 0.21

IFNγ

5.63 ± 1.84

1.08 ± 0.13

0.74 ± 0.10

4.78 ± 2.26

3.14 ± 2.27

0.97 ± 0.68

IGF-1

11.1 ± 2.81

1.09 ± 0.02

1.93 ± 0.46

9.93 ± 0.23

1.17 ± 0.07

1.24 ± 0.17

  1. Where indicated, cultures were treated with 5 μM Aβ, or 5 μM Aβ plus 400 nM PJ34 for 24 hours. Basal concentrations are pg/ml, normalized to microglial protein concentration; mean ± SEM. * p < 0.05 for comparison between wt and PARP-1 -/-. Changes induced by the designated treatments are expressed relative to the basal concentration from each of 4 independent experiments (means ± SEM). # p < 0.05 for comparison between basal concentration and Aβ treatment. † p < 0.05 for comparisons against the Aβ treated wild-type cells (ANOVA with Dunnett's post-test). Differences were not statistically significant when corrected for comparisons between the 7 cytokines analyzed. IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12(p40 &p70), IL-13, IL-17 and GM-CSF were also measured but remained below calibration limits.
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Journal of Neuroinflammation

ISSN: 1742-2094