Table 1 Gene-specific real time RT-PCR primer sequences.
Gene | Sequence (5'-3') | |
|---|---|---|
ß-actin | forward | TGACAGGATGCAGAAGGAGA |
reverse | GCTGGAAGGTGGACAGTGAG | |
CD11b | forward | TCACCCTCAAGGGCAACCTATC |
reverse | AGGGCAAACGCAGAGTCATTAAAC | |
CD45 | forward | TCCCAGCAGACAGGGTTGTTC |
reverse | GTCCATTCTGGGCGGGATAG | |
COX-2 | forward | GTGTGCGACATACTCAAGCAGGA |
reverse | TGAAGTGGTAACCGCTCAGGTG | |
GAT2 | forward | CCATCTTGGGCTTCATGTCTCA |
reverse | CAGCTGGGACAAAGGCATCA | |
GFAP | forward | ACCAGCTTACGGCCAACAGTG |
reverse | TGTCTATACGCAGCCAGGTTGTTC | |
IL-1ß | forward | TCCAGGATGAGGACATGAGCAC |
reverse | GAACGTCACACACCAGCAGGTTA | |
IL-6 | forward | CCACTTCACAAGTCGGAGGCTTA |
reverse | GCAAGTGCATCATCGTTGTTCATAC | |
iNOS | forward | GGAATGGAGACTGTCCCAGCA |
reverse | GTCATGAGCAAAGGCGCAGA | |
Heme oxygenase-1 | forward | TGCAGGTGATGCTGACAGAGG |
reverse | TGTCTGGGATGAGCTAGTGCTGA | |
TNF-α | forward | AAGCCTGTAGCCCACGTCGTA |
reverse | GGCACCACTAGTTGGTTGTCTTTG |