Figure 4

Reduced proinflammatory gene expression in C/EBPβ ^-/- mixed glial cultures. Expression of pro-inflammatory (NOS2, IL-1β, IL-6 and TNFα) and anti-inflammatory (IL-4 and TGFβ1) genes in C/EBPβ^+/+ (white bars) and C/EBPβ^-/- (black bars) mixed glial cultures. Cultures were treated with LPS or LPS+IFNγ for 6 h and then mRNA levels were analyzed by qPCR. In wild type cultures LPS and LPS+IFNγ induce expression of the four pro-inflammatory genes studied but do not affect mRNA levels of the anti-inflammatory genes IL-4 and TGFβ1. Absence of C/EBPβ results in significant decreases in LPS-induced expression of NOS2 and in LPS+IFNγ-induced expression of NOS2 and IL-1β. Results are expressed as relative fold units of ΔΔCt value between gene of interest and actin + Rn18s as reference genes. Two-way ANOVA, followed by Bonferroni's test was applied. *p < 0.05, ***p < 0.001 compared to respective C/EBPβ^+/+ condition. ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 compared to respective control. ^%p < 0.05; ^%%%p < 0.001 compared to respective LPS condition.