Table 2 C/EBPβ binding sites and primers used in quantitative ChIP assay.
Target Gene | C/EBPβ binding site sequence (5→3') Consensus: ATTGCGCAAT | Genomic localization respect to ATG | Primer forward (5→3') | Primer reverse (5→3') |
|---|---|---|---|---|
NOS2 | ggagTGaaGCAATga | -892/-907 | TTATgAgATgTgCCCTCTgC | CCACCTAAggggAACAgTgA |
IL1β | tgtgTgaaGaAAgaa | -16/-31 | TCAggAACAgTTgCCATAgC | AgACCTATACAACggCTCCT |
IL6 | gTttCCAATcagccc | -173/-188 | gTTgTgATTCTTTCgATgCT | ggAATTgACTATCgTTCTTg |
TNFα | agggTTtgGaAAgtt | -336/-351 | TCTCATTCAACCCTCggAAA | CACACACACCCTCCTgATTg |
IL10 | aggATTGaGaAATaa | -463/-448 | TgACTTCCgAgTCAgCAAgA | AgAggCCCTCATCTgTggAT |