Figure 4

PAK1 and IL-1RI modulation following traumatic stress. Rats were killed 1, 3, or 7 days after traumatic stress (n = 5 for each time point), and frontal cortex homogenates were prepared. Immunoprecipitation was used to analyze alterations of PAK1 and IL-RI interaction. The immunoprecipitation antibody was anti-IL-1RI and the immunoblotting antibody was anti-PAK1 (A). Panel B depicts quantitative analysis of A. Data are presented as percentage of control, with the density of PAK1 in the control group (without operation) set at 100%. Values represent mean ± SD for 3 independent experiments. *P <0.05 vs Con. A lipid raft preparation was prepared to determine subcellular distribution of IL-1RI. Western blot analysis was used to detect IL-1RI expression in fractions 3-11, and GM-1 immunopositive fractions were identified as lipid raft fractions (C). Con: control; T1, 3: 1, 3 days after trauma.