Figure 6

PAK1 signaling modulation in traumatized rats. Rats were subjected to surgical trauma, and 3 days later some of these rats were injected icv with adenovirus expressing either the dominant-negative (DN) form of c-Src or the equivalent form of constitutively active (CA) c-Src. Thus, 4 groups of rats were created: Controls (rats with no trauma), T3 (rats killed 3 days after trauma), T3+DN c-Src (rats treated with DN c-Src 3 days after trauma and killed 72 hours later), and T3+CA c-Src (rats treated with CA c-Src 3 days after trauma and killed 72 hours later) (n = 5 for each group). Homogenates of frontal cortex were prepared and assessed for microRNA222 (A) and PAK1 (B) expression using real-time PCR and western blot, respectively. The interaction of PAK1 and IL-RI was assessed by immunoprecipitation (C, D). Data are presented as percentage of control. Values represent mean ± SD for 3 independent experiments. *P <0.05 vs Con. Con: control; T3: 3 days after trauma.