Figure 1

SDF1 and CXCR4 expression in the normal spinal cord. SDF1 is expressed throughout the dCST (a-g) a-b, Cross sections of SDF1-GFP mouse spinal cords show SDF1 in the meninges as well as in the dCST. Rhodamine labeled dextran injection into the sensorimotor cortex shows co-label of SDF1 and dextran dye confirming presence of SDF1-GFP in the dCST (c-g)(red:biotin, green:SDF1-EGFP, blue:Hoechst). e-g, Magnified images of the boxed area of c and d further demonstrating co-label of SDF1 and dCST label. h. The cognate receptor of SDF1, CXCR4, is found only in the ependymal layer (green:CXCR4-EGFP, blue:Hoechst). Scale bars a: 200 μm, b-d: 50 μm, h:20 μm