Figure 2

5 weeks post SCI, SDF1 is found in the dCST rostral to the injury and inside the lesion epicenter. Thoracic cross sections demonstrate SDF1-GFP in the dCST rostral (a, c), but not caudal (b, d) to the injury, however, the SDF1 is present in the meninges throughout (a-d)(green:SDF1-EGFP, blue:Hoechst). A longitudinal section that does not contain the dCST shows some migrating cells expressing SDF1, but most are trapped within the lesion epicenter by the GFAP+ scar (e, f). Longitudinal sections containing the dCST show the end of the tract at a cavitation noted by the high density of nuclei and the GFAP+ scar around its rim (g, h)(red:GFAP, green:SDF1-EGFP, blue:Hoechst) Scale bars: a-b, e: 200 μm, c-d, h: 50 μm, f: 25, g: 100 μm.