CXCR4-GFP cells in the ependymal layer do not have neural progenitor markers. The CXCR4-GFP cells in the ependymal layer have long extended processes, but do not colocalize with GFAP+ processes at 24 hours post injury (a-b) or at 2 weeks (c-d)(red:GFAP, green:CXCR4-EGFP, blue:Hoechst). At 2 weeks, Nestin+ processes also extend from the ependymal layer, but do not colocalize with CXCR4-GFP(e-h). Occasionally, however, the Nestin+ processes are in close apposition to CXCR4-GFP processes (f) (red:Nestin, green:CXCR4-EGFP, blue:Hoechst). Scale bars: a-d: 20 μm, e-h: 50 μm.