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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis

Figure 1

Denaturing ELISA. Comparison of rhMOG118 coated under physiological conditions in PBS (black bars), after partial denaturing of rhMOG118 by incubation with 8 M urea (light grey bars) and after irreversibly completely denaturing by incubation with 8 M urea, 10 mM DTT, 25 mM IDAA (dark grey bars). Results are expressed as binding ratios as described in the methods section. Anti-His denotes a mAb specific for 6-mer histidine peptide tag; #1139 is a human serum serving as positive control in the quantitative ELISA.

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