Figure 1From: Conformational epitopes of myelin oligodendrocyte glycoprotein are targets of potentially pathogenic antibody responses in multiple sclerosis Denaturing ELISA. Comparison of rhMOG118 coated under physiological conditions in PBS (black bars), after partial denaturing of rhMOG118 by incubation with 8 M urea (light grey bars) and after irreversibly completely denaturing by incubation with 8 M urea, 10 mM DTT, 25 mM IDAA (dark grey bars). Results are expressed as binding ratios as described in the methods section. Anti-His denotes a mAb specific for 6-mer histidine peptide tag; #1139 is a human serum serving as positive control in the quantitative ELISA.Back to article page