Systemic inflammatory challenges compromise survival after experimental stroke via augmenting brain inflammation, blood- brain barrier damage and brain oedema independently of infarct size

Table 1 Primers used for real-time PCR

	FORWARD	REVERSE	R²	EFF (%)
GAPDH	TGACGTGCCGCCTGGAGAAA	AGTGTAGCCCAAGATGCCCTTCAG	0,996	91,7
ACTIN-β	CGTAAAGACCTCTATGCCAA	GCGCAAGTTAGGTTTTGTC	0,988	92,5
IL-1β	GCCTCGTGCTGTCGGACCCA	TGAGGCCCAAGGCCACAGGT	0,953	98,1
IL-1α	CCATAACCCATGATCTGGAAGAG	GCTTCATCAGTTTGTATCTCAAATCAC	0,998	104,0
IL-17α	CCTGGCGGCTACAGTGAAG	GGAAGTCCTTGGCCTCAGTGT	0,959	95,8
IL-6	CAGTTGCCTTCTTGGGACTGA	GGGAGTGGTATCCTCTGTGAAGTCT	0,959	108,0
IL-10	AGTGAGAAGCTGAAGACCCTCAGG	TTCATGGCCTTGTAGACACCTTGGT	0,970	107,2
G-CSF	TGCCCAGAGGCGCATGAAGC	GGGGAACGGCCTCTCGTCCT	0,997	102,5
MCP-1	CCAGCACCAGCACCAGCCAA	TGGATGCTCCAGCCGGCAAC	0,967	94,8
TNFα	CAGCCGATGGGTTGTACCTT	GGCAGCCTTGTCCCTTGA	0,985	102,4
KC	GAGCTGCGCTGTCAGTGCCT	CAAGGCAAGCCTCGCGACCA	0,997	105,2

Primers used for real-time PCR (comparative CT experiments) were designed by the Primer Express 3.0 program. Oligonucleotide primer sequences, R2 (regression coefficient) and efficiency (eff %) of the PCR reactions are listed for all primer pairs.