Effects of antibodies to IL-6 and GM-CSF on LPS-induced changes in the permeability of BMECs to 131
I-HIV-1 (A and C) and TEER (B and D). LPS (100 μg/mL) was added to the luminal chamber. In panels A and B, antibodies to IL-6 or GM-CSF were added to the luminal chamber. In panels C and D, antibodies to IL-6 or GM-CSF were added to the abluminal chamber. After 4 hr of incubation, transport study was performed. In panels A and C, results are expressed as % of control. Control values were 1.52 ± 0.16 × 10-5 and 1.52 ± 0.05 × 10-5 cm/min (A and C, respectively). Values are means ± SEM (n = 9-15). *P < 0.05, ***P < 0.001, significant differences from control. #
P < 0.05, ##
P < 0.01, significant differences from LPS (100 μg/mL).