HSP27 is present at the phagocytic cup. Representative confocal photomicrographs were taken to assess the role of LPS on formation of the phagocytic cup. Untreated microglial cells (left panel) and cells treated with LPS (100 ng/ml) (right panel) revealed formation of a cup-shaped structure engulfing an opsonized bead (open arrowhead). LPS treatment induced increased labeling of phosphorylated HSP27 throughout the cell cytoplasm and nucleus as well as in the cup. Cells were stained for phosphorylated HSP27 (in green), actin (in red) and Hoechst 33342 (nuclei in blue). Opsonized beads are labeled in cyan blue. Scale bar 10 μm.