Figure 6

Injured VLWM shows evidence of persistent inflammation. Double immunofluorescence analysis of infiltrated CD3+ T-cells (green) using GFAP (red) antibody to delineate CNS parenchyma within spinal cords of mice evaluated by DTI (A). T cells were absent on spinal cords from naïve mice while some CD3+ lymphocytes were detected on CCX771-treated mice. However, these CD3+ T-cells were mostly restricted to meningeal spaces and within GFAP+ parenchyma. Vehicle- and saline-treated mice showed a higher infiltration of CD3+ lymphocytes within GFAP+ parenchyma. As observed with LFB, To-Pro-3 nuclear staining (blue) is more evident in the 5 mg/kg CCX771-, vehicle- and saline-treated mice. (A 63X magnification, scale bar = 25 μm). Statistical analysis shows significant differences in CD3+ pixel ratios within ROI's (parenchyma and meninges) among treatment groups (B results are expressed as mean CD3+ pixels per ROI ± SEM. Two-way ANOVA P values summary: interaction F = 2.49, P = 0.0347; antagonist treatment F = 13.52, P < 0.0001; area of CNS (parenchyma or meninges) F = 52.86, P < 0.0001).