Journal of Neuroinflammation

Table 1 Oligonucleotide primer sets used for quantitative RT-PCR of brain mRNA samples after cryolesion brain injury.

From: Increased circulating leukocyte numbers and altered macrophage phenotype correlate with the altered immune response to brain injury in metallothionein (MT) -I/II null mutant mice

Primer		Sequence (5' - 3')	Accession No.
GAPDH	Fwd	CCCAGAAGACTGTGGATGG	NM_008084.2
	Rev	GGATGCAGGGATGATGTTCT
IFN-γ	Fwd	ACTGGCAAAAGGATGGTGAC	NM_008337.3
	Rev	GACCTGTGGGTTGTTGACCT
IL-4	Fwd	TCAACCCCCAGCTAGTTGTC	NM_021283.2
	Rev	TCTGTGGTGTTCTTCGTTGC
Ym1	Fwd	ACAATTTAGGAGGTGCCGTG	NM_009892.2
	Rev	CCAGCTGGTACAGCAGACAA
MT-I	Fwd	GCTGTCCTCTAAGCGTCACC	NM_013602.3
	Rev	AGGAGCAGCAGCTCTTCTTG
MT-II	Fwd	CAAACCGATCTCTCGTCGAT	NM_008630.2
	Rev	AGGAGCAGCAGCTTTTCTTG

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ISSN: 1742-2094

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