Figure 1

Slices selection and tissue sampling for neuronal counts and quantification of immunostained area. Fields for neuronal counts, TUNEL evaluation and quantification of stained area were positioned within the ischemic territory at defined distances (see methods for details). The regions sampled pertained to the ischemic territory (yellow) at the time points considered (6 h, 12 h, 24 h, 48 h and 7d).