Coexpression of Ym1 (red) and CD206 (green) at 24 h (A-C-D) and 7d (B-E-F) after pMCAO. At 24 h and 7d after ischemia Ym1 positive cells co-label with CD206 positive cells in the ischemic core (A-B) Bars: 20 μm. High magnification (C-E) and 3D rendering (D-F) show coexpression of markers with the same cell nucleus (blu). Bar: 5 μm. Consistent with the observation that neither Ym1 cells nor CD206 cells are present in the border zone (Figure 4) no immunostaining for these markers could be observed at neither time points in this area (data not shown). Data are representative of 3 independent experiments.