Roles for p38-MAPK, ERK, and JNK in elevation of ApoE expression by IL-1β, Aβ
, sAPP, and glutamate. Cultures of primary cortical neurons or NT2 cells were treated for 20 h with A: IL-1β (30 ng/ml), B: Aβ1-42 (10 μM), C: sAPP (30 nM), D: or glutamate (50 μM). In parallel cultures, these agents were preceded by 5 μM of an inhibitor of p38-MAPK (SB), the ERK pathway (U0126), or JNK (JNKin). Lysates were examined by western blot analysis of full-length ApoE, and autoradiographs were quantified by densitometry (bar graphs). Values represent mean ± SEM (n = 4); *p < 0.05, **p < 0.01.