Apolipoprotein E expression is elevated by interleukin 1 and other interleukin 1-induced factors

Table 1 RT-PCR primers

Gene	Sequences	Annealing temp. (°C)	Cycle no.
Gel-based PCR, rat
ApoE	F: TGT TGG TCC CAT TGC TGA CAG GAT	60	25
	R: TGG TGT TTA CCT CGT TGC GGT ACT
βAPP	F: CCC TGA CGC AGT CGA CAA GT	61	25
	R: TGT CAT AAC CTG GGA CCG GAT
GAPDH	F: CAA CGG ATT TGG CCG TAT TG	61	25
	R: TGG GGG TAG GAA CAC GGA A
ICE	F: ACA AAG AAG GTG GCG CAT TTC	61	28
	R: CCT TGT TTC TCT CCA CGG C
IL-1α	F: GAG TCA ACT CAT TGG CGC TTG	60	27
	R: GGG CTG ATT GAA ACT TAG CCG
IL-1β	F: TGA CTC GTG GGA TGA TGA CG	61	27
	R: CTG GAG ACT GCC CAT TCT CG
TNF	F: GCA CAG AAA GCA TGA TCC GAG	64	28
	R: CCT GGT ATG AAG TGG CAA ATC G
Gel-based PCR, human
ApoE	F: TTC TGT GGG CTG CGT TGC TG	60	25
	R: TAC ACT GCC AGG CGC TTC TG
GAPDH	F: AGG TCG GAG TCA ACG GAT TTG	61	25
	R: TGG CAG GTT TTT CTA GAC GGC
Real-time PCR (rat)
ApoE	F: CTG GTT CGA GCC GCT AGT G	60	N.A.
	R: CCT GTA TCT TCT CCA TTA GGT TTG C
18S	F: TTC GAA CGT CTG CCC TAT CAA	60	N.A.
	R: ATG GTA GGC ACG GCG ACT A

ISSN: 1742-2094