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Table 1 RT-PCR primers

From: Apolipoprotein E expression is elevated by interleukin 1 and other interleukin 1-induced factors

Gene Sequences Annealing
temp. (°C)
Cycle no.
Gel-based PCR, rat
ApoE F: TGT TGG TCC CAT TGC TGA CAG GAT 60 25
  R: TGG TGT TTA CCT CGT TGC GGT ACT   
βAPP F: CCC TGA CGC AGT CGA CAA GT 61 25
  R: TGT CAT AAC CTG GGA CCG GAT   
GAPDH F: CAA CGG ATT TGG CCG TAT TG 61 25
  R: TGG GGG TAG GAA CAC GGA A   
ICE F: ACA AAG AAG GTG GCG CAT TTC 61 28
  R: CCT TGT TTC TCT CCA CGG C   
IL-1α F: GAG TCA ACT CAT TGG CGC TTG 60 27
  R: GGG CTG ATT GAA ACT TAG CCG   
IL-1β F: TGA CTC GTG GGA TGA TGA CG 61 27
  R: CTG GAG ACT GCC CAT TCT CG   
TNF F: GCA CAG AAA GCA TGA TCC GAG 64 28
  R: CCT GGT ATG AAG TGG CAA ATC G   
Gel-based PCR, human
ApoE F: TTC TGT GGG CTG CGT TGC TG 60 25
  R: TAC ACT GCC AGG CGC TTC TG   
GAPDH F: AGG TCG GAG TCA ACG GAT TTG 61 25
  R: TGG CAG GTT TTT CTA GAC GGC   
Real-time PCR (rat)
ApoE F: CTG GTT CGA GCC GCT AGT G 60 N.A.
  R: CCT GTA TCT TCT CCA TTA GGT TTG C   
18S F: TTC GAA CGT CTG CCC TAT CAA 60 N.A.
  R: ATG GTA GGC ACG GCG ACT A