Influence of VEGF inhibition after PDT. (A-D) Hematoxylin-eosin staining of a retinal section 7 days after PDT with VEGF inhibition. Damage to the photoreceptor cell layer was obvious when VEGFR1 Fc was injected into the eye immediately after PDT (B-D). (E-H) TUNEL (red) and Hoechst (blue) stainings of retinal sections 3 days after PDT with VEGF inhibition. TUNEL-positive cells increased when VEGFR1 Fc was injected after PDT (F-H). The thickness of photoreceptor cell layer in the center of the lesion was measured (I) and the number of TUNEL-positive cells were counted (J). The effects were dose-dependent. Scale bar, 100 μm. *p < 0.05, **p < 0.01.