Influence of VEGF inhibition on Akt and BAX. (A-D) Immunoblot analyses. One day after PDT with vehicle injection, pAkt levels increased, but levels were decreased by VEGF inhibition with injection of VEGFR1 Fc (2 μg/μl) into the eye immediately after PDT (A,B). BAX levels in the retina were not changed by PDT when vehicle was injected, but increased when VEGFR1 Fc (2 μg/μl) was injected immediately after PDT (C,D). (E) Immunohistochemistry. Three days after PDT, pAkt was observed in photoreceptor cells, in cell bodies and outer segments (arrows), of the irradiated area of the vehicle-treated retina, but little staining was observed in the VEGFR1 Fc (2 μg/μl)-treated retina. pAkt, phosphorylated Akt, ONL, outer nuclear layer, OS, outer segment. *p < 0.05, **p < 0.01.