Influence of Akt inhibition after PDT. (A-D) Immunoblot analyses. pAkt levels decreased (A,B) and BAX levels increased (C,D) in retina 1 day after a PI3K inhibitor (LY294002, 10 μM) was injected into the eye. (E-G) TUNEL (red) and Hoechst (blue) stainings 3 days after PDT. LY294002 injection increased the number of TUNEL-positive cells in the irradiated area. pAkt, phosphorylated Akt. Scale bar, 50 μm. *p < 0.05.