Figure 4
CNS-infiltrating mononuclear cells from TMEV-infected NLM and TLR3KO-SJL mice. (A) Overall numbers of CNS-infiltrating mononuclear cells in the CNS of TMEV-infected NLM and TLR3KO-SJL at 7, 21, and 80 DPI were shown. The values given are means ± standard deviations of 2-3 independent experiments derived from pooled cells of 3-4 mice per group. (B) Levels of T cells (CD4+ and CD8+), macrophages (CD11b+ CD45high) and granulocytes (Ly6G/6C+ and CD45+) were assessed using flow cytometry. Numbers in the FACS plots represent percentages in the CNS of TMEV-infected NLM and TLR3KO-SJL at 7 and 21 dpi. (C) Cytokine mRNA expression levels in the CNS of NLM and TLR3KO-SJL at 7 and 21 dpi were analyzed by quantitative PCR. Data are expressed by fold induction after normalization to the GAPDH mRNA levels. The values given are means ± standard deviations of triplicates. Statistically significant differences were indicated with asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.001). A representative result of three separate experiments using three mice per group was shown here.