CD3, GFAP, DAPI Immunostaining and quantitative real-time PCR analysis of T cells and related cytokines in IRF7-KO and WT mice with EAE. CD3+ T cells were dispersed more diffusely in the spinal cord of IRF7-deficient mice with EAE (B) compared to the more focal infiltration pattern in WT spinal cord (A). IRF7 deficiency had no apparent effect on GFAP+ cells (astrocytes). A-B) original magnification 20×. C-E). CD3ε (C), IFN-γ (D), and IL-17 (E) gene expression in the CNS of IRF7-deficient (n = 6-7) and WT mice (n = 5-7) were calculated and normalized to 18s rRNA.