Figure 7

Activation of c-Jun N terminal kinase (JNK) signaling pathway. Proteins extracted from the ischemic hemisphere of wild type and MCPIP1 knockout mice undergoing ischemia 30 min followed by 30 and 90 min reperfusion separately. The samples were subjected to Western blot analysis with antibodies as indicated. (A) A representative western blot shows protein levels of JNK phosphorylation. (B) Densitometric analysis was used to quantify p-JNK protein levels versus total JNK in 3 independent western blots and the data are expressed as the normalized folds with respect to sham. Values represent mean ± SD, *p < 0.05 versus sham-treated control. (C) A representative western blot shows protein levels of c-jun phosphorylation. (D) Densitometric analysis was used to quantify p-c-cun protein levels versus total c-jun in 3 independent western blots and the data are expressed as the normalized folds with respect to sham. Values represent mean ± SD, *p < 0.05 versus sham-treated control.