Figure 1
From: Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro
Recombinant NT-3 stimulates neurite density and length of organotypic brain slices. The neurotrophins NGF, BDNF, NT-3 and NT-4 (500 ng/ml) were added to the culture medium immediately after preparation of the organotypic brain slices. NT-3, but not the other neurotrophins significantly increases neurite density (A), neurite length (B), the amount of slices reaching the maximum outgrowth (C) and the amount of slices reaching the maximum length (D). E + F: representative photomicrograph showing the increase in outgrowth of NT-3 treated EC slices compared to control. n = 50 slices. A + B: *: Statistically significant difference to control; p < 0.05 (Mann Whitney U test). C + D: *: Statistically significant difference; p < 0.05 (Chi-square analysis). EC = enthorinal cortex. Arrows indicate outgrowing neuritis. Scale bar: 100 μm.