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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro

Figure 2

IL-1β stimulates neurite density and length in organotypic brain slices. A dose-response curve revealed that high doses of IL-1β (500 ng/ml) added to the culture medium, stimulate neurite density (A) of organotypic brain slices and the amount of slices reaching the maximum outgrowth (C). A lower dose (50 ng/ml) is still able to stimulate the average length of neuritis (B) but neither 500 ng/ml nor 50 ng/ml of IL-1β were able to significantly increase the amount of slices presenting maximum length (D). E + F: representative photomicrograph showing the increase in outgrowth of IL-1β treated EC compared to control. n = 17 slices. A + B: *: Statistically significant difference to control; p < 0.05 (Mann Whitney U test). C + D: *: Statistically significant difference; p < 0.05 (Chi-square analysis) EC = enthorinal cortex. Arrows indicate neuritis. Scale bar: 100 μm.

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