Skip to main content
Figure 4 | Journal of Neuroinflammation

Figure 4

From: Interleukin-1 beta and neurotrophin-3 synergistically promote neurite growth in vitro

Figure 4

Neurite outgrowth is independent on endogenous IL-1beta and is synergistically stimulated by combined application of NT-3 and IL-1beta. A + B: Neurite outgrowth (neurite density A and neurite length B) was not influenced in the absence of endogenous IL-1β in IL-1β-deficient mice. Heterozygous IL-1β-deficient and wildtype mice served as controls. n: 50 slices. C + D: The combined administration of NT-3 and IL-1β shows only a slight increase in neurite density and length, if compared to single treatments. n = 84 slices. *: Statistically significant difference to control; p < 0.05 (Mann Whitney U test). Error bars represent SEM. E: Chi-square analysis reveals a significant difference in the frequency of brain slices with maximal outgrowth between single treatments with NT3 or IL-1β and the combined administration of both factors. Particularly double treatment presents a significant increase in the number of slices reaching maximum density if compared to control and single treatment with IL-1β, and in length if compared to single treatment with NT3. Additionally, combination of the two factors is also characterized by a significantly higher number of slices which hit the maximum in both parameters, if compared to control condition or single treatment with NT3 (to increase the readability of the graph only the significances relative to the double treatment have been included). *: Statistically significant difference; p < 0.05 (Chi-square).

Back to article page