Figure 2

Microglial genes that are upregulated (A) or downregulated (B) by Ad-IRF3 (Q-PCR). Primary human microglial cultures were exposed to medium alone (Ctr), Ad-GFP (GFP) or Ad-IRF3 (IRF3) at 500 multiplicity of infection (moi) for 48 h, then stimulated with IL-1β and IFNγ at 10 ng/ml each for 16 h. Q-PCR analysis was performed using PBDA as a control. Y-axis denote fold changes calculated as described in the Methods section, then normalized to the control level (without cytokine treatment). Note varying Y-axis scales. All samples were tested in triplicate, and p-values were calculated by t-test (GFP vs. IRF3) using GraphPad Prism 5. * p < 0.05, ** p < 0.01, *** p < 0.001.