Figure 4

Ad-IRF3 modulated microglial cytokine production (ELISA). Microglia were grown in 96-well plates and left uninfected (Ctr) or infected with Ad-GFP or Ad-IRF3 for 48 h, and treated with IL-1β/IFNγ (10 ng/ml each), IL-1β alone (10 ng/ml) or LPS (100 ng/ml) for 24 h. Culture supernatants (IL-1ra, IP-10, and IL-8) or cell lysates (IL-1β) were analyzed for cytokine levels by ELISA as described in the Methods section. All samples were tested in triplicate, and p-value was calculated by t-test (GFP vs. IRF3). * p < 0.05, ** p < 0.01, ***p < 0.001.